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Nup358 integrates nuclear envelope breakdown with kinetochore assembly

Salina, D; Enarson, P; Rattner, JB; Burke, B

Nup358 integrates nuclear envelope breakdown with kinetochore assembly Thumbnail


Authors

D Salina

P Enarson

JB Rattner

B Burke



Abstract

Nuclear envelope breakdown (NEBD) and release of condensed chromosomes into the cytoplasm are key events in the early stages of mitosis in metazoans. NEBD involves the disassembly of all major structural elements of the nuclear envelope, including nuclear pore complexes (NPCs), and the dispersal of nuclear membrane components. The breakdown process is facilitated by microtubules of the mitotic spindle. After NEBD, engagement of spindle microtubules with chromosome-associated kinetochores leads to chromatid segregation. Several NPC subunits relocate to kinetochores after NEBD. siRNA-mediated depletion of one of these proteins, Nup358, reveals that it is essential for kinetochore function. In the absence of Nup358, chromosome congression and segregation are severely perturbed. At the same time, the assembly of other kinetochore components is strongly inhibited, leading to aberrant kinetochore structure. The implication is that Nup358 plays an essential role in integrating NEBD with kinetochore maturation and function. Mitotic arrest associated with Nup358 depletion further suggests that mitotic checkpoint complexes may remain active at nonkinetochore sites.

Citation

Salina, D., Enarson, P., Rattner, J., & Burke, B. (2003). Nup358 integrates nuclear envelope breakdown with kinetochore assembly. Journal of Cell Biology, 162(6), 991-1001. https://doi.org/10.1083/jcb.200304080

Journal Article Type Article
Publication Date Sep 1, 2003
Deposit Date Aug 29, 2008
Publicly Available Date Mar 28, 2024
Journal Journal of Cell Biology
Print ISSN 0021-9525
Electronic ISSN 1540-8140
Publisher Rockefeller University Press
Peer Reviewed Peer Reviewed
Volume 162
Issue 6
Pages 991-1001
DOI https://doi.org/10.1083/jcb.200304080
Keywords Nuclear pore complex, Kinetochore, Mitosis, Nup358, Mitotic checkpoint.

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