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Photo-isomerization of the isolated photoactive yellow protein chromophore: what comes before the primary step?

Anstöter, Cate S.; Curchod, Basile F.E.; Verlet, Jan R.R.

Photo-isomerization of the isolated photoactive yellow protein chromophore: what comes before the primary step? Thumbnail


Authors

Cate S. Anstöter



Abstract

Photoactive proteins typically rely on structural changes in a small chromophore to initiate a biological response. While these changes often involve isomerization as the “primary step”, preceding this is an ultrafast relaxation of the molecular framework caused by the sudden change in electronic structure upon photoexcitation. Here, we capture this motion for an isolated model chromophore of the photoactive yellow protein using time-resolved photoelectron imaging. It occurs in <150 fs and is apparent from a spectral shift of ∼70 meV and a change in photoelectron anisotropy. Electronic structure calculations enable the quantitative assignment of the geometric and electronic structure changes to a planar intermediate from which the primary step can then proceed.

Citation

Anstöter, C. S., Curchod, B. F., & Verlet, J. R. (2022). Photo-isomerization of the isolated photoactive yellow protein chromophore: what comes before the primary step?. Physical Chemistry Chemical Physics, 24(3), 1305-1309. https://doi.org/10.1039/d1cp05259d

Journal Article Type Article
Acceptance Date Dec 23, 2021
Online Publication Date Dec 23, 2021
Publication Date Jan 21, 2022
Deposit Date Mar 15, 2022
Publicly Available Date Mar 29, 2024
Journal Physical Chemistry Chemical Physics
Print ISSN 1463-9076
Electronic ISSN 1463-9084
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 24
Issue 3
Pages 1305-1309
DOI https://doi.org/10.1039/d1cp05259d

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