Denny, P. W. and Field, M. C. and Smith, D. F. (2001) 'GPI-anchored proteins and glycoconjugates segregate into lipid rafts in Kinetoplastida.', FEBS letters., 491 (1-2). pp. 148-153.
The plasma membranes of the divergent eukaryotic parasites, Leishmania and Trypanosoma, are highly specialised, with a thick coat of glycoconjugates and glycoproteins playing a central role in virulence. Unusually, the majority of these surface macro-molecules are attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor. In mammalian cells and yeast, many GPI-anchored molecules associate with sphingolipid and cholesterol-rich detergent-resistant membranes, known as lipid rafts. Here we show that GPI-anchored parasite macro-molecules (but not the dual acylated Leishmania surface protein (hydrophilic acylated surface protein) or a subset of the GPI-anchored glycoinositol phospholipid glycolipids) are enriched in a sphingolipid/sterol-rich fraction resistant to cold detergent extraction. This observation is consistent with the presence of functional lipid rafts in these ancient, highly polarised organisms.
|Keywords:||Lipid raft, Kinetoplastida, Glycosylphosphatidylinositol anchor, Sphingolipid, Sterol.|
|Full text:||PDF - Accepted Version (521Kb)|
|Publisher Web site:||http://dx.doi.org/10.1016/S0014-5793(01)02172-X|
|Record Created:||18 Feb 2009|
|Last Modified:||26 Aug 2011 14:25|
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