Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

GPI-anchored proteins and glycoconjugates segregate into lipid rafts in Kinetoplastida.

Denny, P.W. and Field, M.C. and Smith, D.F. (2001) 'GPI-anchored proteins and glycoconjugates segregate into lipid rafts in Kinetoplastida.', FEBS letters., 491 (1-2). pp. 148-153.

Abstract

The plasma membranes of the divergent eukaryotic parasites, Leishmania and Trypanosoma, are highly specialised, with a thick coat of glycoconjugates and glycoproteins playing a central role in virulence. Unusually, the majority of these surface macro-molecules are attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor. In mammalian cells and yeast, many GPI-anchored molecules associate with sphingolipid and cholesterol-rich detergent-resistant membranes, known as lipid rafts. Here we show that GPI-anchored parasite macro-molecules (but not the dual acylated Leishmania surface protein (hydrophilic acylated surface protein) or a subset of the GPI-anchored glycoinositol phospholipid glycolipids) are enriched in a sphingolipid/sterol-rich fraction resistant to cold detergent extraction. This observation is consistent with the presence of functional lipid rafts in these ancient, highly polarised organisms.

Item Type:Article
Keywords:Lipid raft, Kinetoplastida, Glycosylphosphatidylinositol anchor, Sphingolipid, Sterol.
Full text:PDF - Accepted Version (521Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1016/S0014-5793(01)02172-X
Record Created:18 Feb 2009
Last Modified:13 Jan 2014 16:52

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitterExport: EndNote, Zotero | BibTex
Usage statisticsLook up in GoogleScholar | Find in a UK Library