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Realtime wavefront sensing in a SPIM microscope, and active aberration tracking

Taylor, Jonathan M.; Saunter, Christopher D.; Bourgenot, Cyril; Girkin, John M.; Love, Gordon D.

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Authors

Jonathan M. Taylor

Christopher D. Saunter

Gordon D. Love



Contributors

Thomas G. Bifano
Editor

Joel Kubby
Editor

Sylvain Gigan
Editor

Abstract

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.

Citation

Taylor, J. M., Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2015). Realtime wavefront sensing in a SPIM microscope, and active aberration tracking. In T. G. Bifano, J. Kubby, & S. Gigan (Eds.), . https://doi.org/10.1117/12.2080061

Conference Name Adaptive Optics and Wavefront Control for Biological Systems
Conference Location San Francisco, CA
Start Date Feb 7, 2015
End Date Feb 12, 2015
Online Publication Date Mar 31, 2015
Publication Date 2015
Deposit Date Feb 13, 2018
Publicly Available Date Mar 29, 2024
Volume 9335
Series ISSN 1605-7422
DOI https://doi.org/10.1117/12.2080061
Public URL https://durham-repository.worktribe.com/output/1145869

Files

Published Conference Proceeding (775 Kb)
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Copyright Statement
Copyright 2015 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.





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