Casson, S. A. and Chilley, P. and Topping, J. F. and Evans, I. M. and Souter, M. and Lindsey, K. (2002) 'The POLARIS gene of Arabidopsis encodes a predicted peptide required for correct root growth and leaf vascular patterning.', Plant cell., 14 (8). pp. 1705-1721.
The POLARIS (PLS) gene of Arabidopsis was identified as a promoter trap transgenic line, showing -glucuronidase fusion gene expression predominantly in the embryonic and seedling root, with low expression in aerial parts. Cloning of the PLS locus revealed that the promoter trap T-DNA had inserted into a short open reading frame (ORF). Rapid amplification of cDNA ends PCR, RNA gel blot analysis, and RNase protection assays showed that the PLS ORF is located within a short (500 nucleotides) auxin-inducible transcript and encodes a predicted polypeptide of 36 amino acid residues. pls mutants exhibit a short-root phenotype and reduced vascularization of leaves. pls roots are hyperresponsive to exogenous cytokinins and show increased expression of the cytokinin-inducible gene ARR5/IBC6 compared with the wild type. pls seedlings also are less responsive to the growth-inhibitory effects of exogenous auxin and show reduced expression of the auxin-inducible gene IAA1 compared with the wild type. The PLS peptide-encoding region of the cDNA partially complements the pls mutation and requires the PLS ORF ATG for activity, demonstrating the functionality of the peptide-encoding ORF. Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins and increases leaf vascularization. We propose that PLS is required for correct auxin-cytokinin homeostasis to modulate root growth and leaf vascular patterning.
|Full text:||Full text not available from this repository.|
|Publisher Web site:||http://www.plantcell.org/cgi/content/abstract/14/8/1705|
|Record Created:||17 May 2007|
|Last Modified:||07 Apr 2010 10:50|
|Social bookmarking:||Export: EndNote, Zotero | BibTex|
|Look up in GoogleScholar | Find in a UK Library|