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Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase

O'Hara, P.; Slabas, AR.; Fawcett, T.

Authors

P. O'Hara

AR. Slabas



Abstract

In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.

Citation

O'Hara, P., Slabas, A., & Fawcett, T. (2001). Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase. FEBS Letters, 488(1-2), 18-22. https://doi.org/10.1016/s0014-5793%2800%2902406-6

Journal Article Type Article
Publication Date Jan 1, 2001
Deposit Date May 17, 2007
Journal FEBS Letters
Print ISSN 0014-5793
Publisher Federation of European Biochemical Societies
Peer Reviewed Peer Reviewed
Volume 488
Issue 1-2
Pages 18-22
DOI https://doi.org/10.1016/s0014-5793%2800%2902406-6
Keywords 3-Oxoacyl-ACP reductase, Fatty acid synthase, [3H]NADPH, Brassica napus.

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