O'Hara, P. and Slabas, A. R. and Fawcett, T. (2001) 'Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase.', FEBS letters., 488 (1-2). pp. 18-22.
In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.
|Keywords:||3-Oxoacyl-ACP reductase, Fatty acid synthase, [3H]NADPH, Brassica napus.|
|Full text:||Full text not available from this repository.|
|Publisher Web site:||http://dx.doi.org/10.1016/S0014-5793(00)02406-6|
|Record Created:||17 May 2007|
|Last Modified:||20 Mar 2010 15:55|
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