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Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase.

O'Hara, P. and Slabas, A. R. and Fawcett, T. (2001) 'Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase.', FEBS letters., 488 (1-2). pp. 18-22.

Abstract

In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.

Item Type:Article
Keywords:3-Oxoacyl-ACP reductase, Fatty acid synthase, [3H]NADPH, Brassica napus.
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1016/S0014-5793(00)02406-6
Record Created:17 May 2007
Last Modified:20 Mar 2010 15:55

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