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Degradation of de-esterified pectin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton

Wu, Y.; Li, X.; Li, Y.; Ma, H.; Chi, H.; Ma, Y.; Yang, J.; Xie, S.; Zhang, R.; Liu, L.; Su, X.; Lv, R.; Khan, A.; Kong, J.; Xiaoping, G.; Lindsey, K.; Min, L.; Zhang, X.

Degradation of de-esterified pectin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton Thumbnail


Authors

Y. Wu

X. Li

Y. Li

H. Ma

H. Chi

Y. Ma

J. Yang

S. Xie

R. Zhang

L. Liu

X. Su

R. Lv

A. Khan

J. Kong

G. Xiaoping

L. Min

X. Zhang



Abstract

The pollen wall exine provides a protective layer for the male gametophyte and is largely composed of sporopollenin, which comprises fatty acid derivatives and phenolics. However, the biochemical nature of the external exine is poorly understood. Here, we show that the male sterile line 1355A of cotton mutated in NO SPINE POLLEN (GhNSP) leads to defective exine formation. The GhNSP locus was identified through map-based cloning and confirmed by genetic analysis (co-segregation test and allele prediction using the CRISPR/Cas9 system). In situ hybridization showed that GhNSP is highly expressed in tapetum. GhNSP encodes a polygalacturonase protein homologous to AtQRT3, which suggests a function for polygalacturonase in pollen exine formation. These results indicate that GhNSP is functionally different from AtQRT3, the latter has the function of microspore separation. Biochemical analysis showed that the percentage of de-esterified pectin was significantly increased in the 1355A anthers at developmental stage 8. Furthermore, immunofluorescence studies using antibodies to the de-esterified and esterified homogalacturonan (JIM5 and JIM7) showed that the Ghnsp mutant exhibits abundant of de-esterified homogalacturonan in the tapetum and exine, coupled with defective exine formation. The characterization of GhNSP provides new understanding of the role of polygalacturonase and de-esterified homogalacturonan in pollen exine formation.

Citation

Wu, Y., Li, X., Li, Y., Ma, H., Chi, H., Ma, Y., …Zhang, X. (2022). Degradation of de-esterified pectin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton. Plant Biotechnology Journal, 20(6), 1054-1068. https://doi.org/10.1111/pbi.13785

Journal Article Type Article
Acceptance Date Jan 22, 2022
Online Publication Date Feb 18, 2022
Publication Date 2022-06
Deposit Date Apr 5, 2022
Publicly Available Date May 31, 2022
Journal Plant Biotechnology Journal
Print ISSN 1467-7644
Electronic ISSN 1467-7652
Publisher Association of Applied Biologists
Peer Reviewed Peer Reviewed
Volume 20
Issue 6
Pages 1054-1068
DOI https://doi.org/10.1111/pbi.13785

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.





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