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Increased Heat Shock Protein 70 Expression Following Toxicant-Mediated Cytotoxicity: A Ubiquitous Marker Of Toxicant Exposure?

Farzaneh, P.; Allameh, A.; Pratt, S.; Moore, N.; Travis, L.; Gottschalg, E.; Kind, C.; Fry, J.

Authors

P. Farzaneh

A. Allameh

S. Pratt

N. Moore

L. Travis

E. Gottschalg

C. Kind

J. Fry



Abstract

The up-regulation of heat shock protein (HSP) expression has been proposed as a general biomarker of cellular protection against various environmental stresses and chemicals. The present study investigated the possibility of using HSP70 up-regulation as a biomarker of toxicant exposure in vitro. Cells of a rat hepatoma cell line (FGC4) were exposed to concentrations of 1,3-dichloroacetone, duroquinone, diquat dibromide, menadione, hydrogen peroxide, cadmium chloride (CdCl2) and sodium (meta)arsenite (NaAsO2) that elicited 20–50% cytotoxicity over a 24-hour period, and HSP70 levels were measured by ELISA. Up-regulation of HSP70 expression was demonstrated following treatment with menadione, CdCl2 and NaAsO2, but not with the other chemicals tested. A shorter exposure time (6 hours) and/or the use of non-toxic concentrations reduced the level of HSP70 up-regulation with menadione, CdCl2 and NaAsO2, but did not uncover any up-regulation with the other chemicals. Although the toxicity of the majority of the chemicals tested is believed to involve an oxidative stress component, the results of this study clearly demonstrate that up-regulation of HSP70 expression cannot be used as a general biomarker of toxicant exposure in vitro.

Citation

Farzaneh, P., Allameh, A., Pratt, S., Moore, N., Travis, L., Gottschalg, E., …Fry, J. (2005). Increased Heat Shock Protein 70 Expression Following Toxicant-Mediated Cytotoxicity: A Ubiquitous Marker Of Toxicant Exposure?. Alternatives to Laboratory Animals, 33(2), 105-110

Journal Article Type Article
Publication Date Apr 1, 2005
Deposit Date Jul 4, 2008
Journal Alternatives to Laboratory Animals
Print ISSN 0261-1929
Publisher Fund for the Replacement of Animals in Medical Experiments (FRAME)
Peer Reviewed Peer Reviewed
Volume 33
Issue 2
Pages 105-110
Keywords Arsenite, Cadmium, Cytotoxicity, Dichloroacetone, Diquat, Duroquinone, Heat shock protein, HSP70, Hydrogen peroxide, Menadione.
Publisher URL http://www.frame.org.uk/atlafn/atlaintro.htm