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Proteomic study of the soluble proteins from the unicellular cyanobacterium Synechocystis sp PCC6803 using automated matrix-assisted laser desorption/ionization-time of flight peptide mass fingerprinting.

Simon, W. J. and Hall, J. J. and Suzuki, I. and Murata, N. and Slabas, A. R. (2002) 'Proteomic study of the soluble proteins from the unicellular cyanobacterium Synechocystis sp PCC6803 using automated matrix-assisted laser desorption/ionization-time of flight peptide mass fingerprinting.', Proteomics., 2 (12). pp. 1735-1742.

Abstract

The unicellular cyanobacteria Synechocystis sp. (PCC6803) has become a model organism for a range of biochemical and molecular biology studies aimed at investigating environmental stress responses. In this study the soluble proteins of Synechocystis were analysed using narrow pH range (pH 4.5-5.5) zoom gels, automated matrix-assisted laser desorption/ionization mass spectrometry acquisition, spectral processing and database searching. The work sets the foundation for investigations of proteomic changes following stress treatment. One hundred and ninety-two protein spots were analysed and 105 proteins identified, of these 37 were novel proteins not previously seen on two-dimensional gels. Proteins involved in amino acid biosynthesis, energy metabolism and protein modification were identified using this fully automated procedure demonstrating that automated acquisition and processing will be a useful tool for proteomic analyses on this organism.

Item Type:Article
Additional Information:
Keywords:Automated matrix-assisted laser desorption/ionization-mass spectrometry analysis, Cyanobacteria, Synechocystis.
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1002/1615-9861(200212)2:12<1735::AID-PROT1735>3.0.CO;2-K
Record Created:18 May 2007
Last Modified:08 Apr 2009 16:32

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