S Muller
The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function
Muller, S; Smertenko, A; Wagner, V; Heinrich, M; Hussey, PJ; Hauser, MT
Authors
A Smertenko
V Wagner
M Heinrich
PJ Hussey
MT Hauser
Abstract
Directional cell expansion in interphase and nuclear and cell division in M-phase are mediated by four microtubule arrays, three of which are unique to plants: the interphase array, the preprophase band, and the phragmoplast. The plant microtubule-associated protein MAP65 has been identified as a key structural component in these arrays [1]. The Arabidopsis genome has nine MAP65 genes, and here we show that one, AtMAP65-3/PLE, locates only to the mitotic arrays and is essential for cytokinesis. The Arabidopsis pleiade (ple) alleles are single recessive mutations, and we show that these mutations are in the AtMAP65-3 gene. Moreover, these mutations cause C-terminal truncations that abolish microtubule binding. In the ple mutants the anaphase spindle is normal, and the cytokinetic phragmoplast can form but is distorted; not only is it wider, but the midline, the region where oppositely oriented microtubules overlap, is unusually expanded. Here we present data that demonstrate an essential role for AtMAP65-3/PLE in cytokinesis in plant cells.
Citation
Muller, S., Smertenko, A., Wagner, V., Heinrich, M., Hussey, P., & Hauser, M. (2004). The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function. Current Biology, 14(5), 412-417. https://doi.org/10.1016/j.cub.2004.02.032
Journal Article Type | Article |
---|---|
Publication Date | Mar 1, 2004 |
Deposit Date | May 18, 2007 |
Journal | Current Biology |
Print ISSN | 0960-9822 |
Publisher | Cell Press |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Issue | 5 |
Pages | 412-417 |
DOI | https://doi.org/10.1016/j.cub.2004.02.032 |
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