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Tracing functionally identified neurones in a multisynaptic pathway in the hamster and rat using herpes simplex virus expressing green fluorescent protein

Pyner, S.; Cleary, J.; McLeish, P.; Buchan, A.; Coote, JH

Authors

J. Cleary

P. McLeish

A. Buchan

JH Coote



Abstract

Using a genetically modified herpes simplex virus encoding green fluorescent protein we sought to establish if this viral modification could be used in transneuronal tracing studies of the sympathetic nervous system. The herpes simplex virus encoding green fluorescent protein was injected into the adrenal medulla of three hamsters and six rats. After a suitable survival period, neurones in the sympathetic intermediolateral cell column of the thoracolumbar spinal cord, rostral ventral medulla and paraventricular nucleus of the hypothalamus were clearly identified by the presence of a green fluorescence in the cytoplasm of the neurones of both species. Thus, herpes simplex virus encoding green fluorescent protein labelled chains of sympathetic neurones in the hamster and rat and therefore has the potential to be used in transneuronal tracing studies of autonomic pathways in these species.

Citation

Pyner, S., Cleary, J., McLeish, P., Buchan, A., & Coote, J. (2001). Tracing functionally identified neurones in a multisynaptic pathway in the hamster and rat using herpes simplex virus expressing green fluorescent protein. Experimental Physiology, 86(6), 695-702. https://doi.org/10.1111/j.1469-445x.2001.tb00034.x

Journal Article Type Article
Publication Date Nov 1, 2001
Deposit Date May 21, 2008
Journal Experimental Physiology
Print ISSN 0958-0670
Electronic ISSN 1469-445X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 86
Issue 6
Pages 695-702
DOI https://doi.org/10.1111/j.1469-445x.2001.tb00034.x


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