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In vivo induction of hair growth by dermal cells isolated from hair follicles after extended organ culture.

Robinson, M. and Reynolds, A. J. and Gharzi, A. and Jahoda, C. A. B. (2001) 'In vivo induction of hair growth by dermal cells isolated from hair follicles after extended organ culture.', Journal of investigative dermatology., 117 (3). pp. 596-604.

Abstract

Successful hair follicle organ culture has been established for some time, but hair growth in vitro is limited and generally terminates prematurely in comparison with in vivo. The reasons why growth stops in culture are as yet unknown. In this investigation, adult rat vibrissa follicles for which growth in culture is limited to about 10 d, were maintained in vitro for a minimum of 20 d after the hair shaft stopped growing. The pattern of fiber growth and long-term follicle pathology reflected the initial hair cycle stage at the time of isolation. Furthermore, there was evidence that a group of follicles put into culture when in late anagen were attempting to cycle in vitro. Microscopy showed that, in spite of widespread pathologic changes to the follicle epithelium, dermal cells in the follicle showed remarkable resilience. Their viability was confirmed when primary cell cultures were established from isolated dermal tissue. These cells labeled positively for -smooth muscle actin, an established marker of hair follicle dermal cell phenotype in vitro. Moreover, isolated dermal tissue induced hair growth when implanted into inactivated hair follicles in vivo. These data confirm that the cessation in hair growth is not due to a loss of the inductive capacity in the dermal component. Long-term organ culture may provide opportunities to investigate factors that are expressed or lost during hair growth cessation. In addition it may be possible to develop this method further to obtain a reliable and predictable model of hair follicle cycling in vitro.

Item Type:Article
Additional Information:
Keywords:Dermal papilla, Dermal sheath, Epithelial-mesenchymal interactions.
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1046/j.0022-202x.2001.01461.x
Record Created:18 May 2007
Last Modified:08 Apr 2009 16:32

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