A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells

van Lith, M.; Karala, A.; Bown, D.; Gatehouse, J.; Ruddock, L.; Saunders, P; Benham, AM.

doi:10.1091/mbc.e07-02-0147

A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells

van Lith, M.; Karala, A.; Bown, D.; Gatehouse, J.; Ruddock, L.; Saunders, P; Benham, AM.

Authors

M. van Lith

A. Karala

D. Bown

J. Gatehouse

L. Ruddock

P Saunders

Professor Adam Benham adam.benham@durham.ac.uk
Professor

Abstract

Glycoprotein folding is mediated by lectin-like chaperones and protein disulfide isomerases (PDIs) in the endoplasmic reticulum (ER). Calnexin and the PDI homologue ERp57 work together to help fold nascent polypeptides with glycans located toward the N-terminus of a protein, whereas PDI and BiP may engage proteins that lack glycans or have sugars toward the C-terminus. In this study, we show that the PDI homologue PDILT is expressed exclusively in post-meiotic male germ cells, in contrast to the ubiquitous expression of many other PDI family members in the testis. PDILT is induced during puberty and represents the first example of a PDI family member under developmental control. We find that PDILT is not active as an oxido-reductase, but interacts with the model peptide -somatostatin and nonnative BPTI in vitro, indicative of chaperone activity. In vivo, PDILT forms a tissue-specific chaperone complex with the calnexin homologue calmegin. The identification of a redox-inactive chaperone partnership defines a new system of testis-specific protein folding with implications for male fertility.

Citation

van Lith, M., Karala, A., Bown, D., Gatehouse, J., Ruddock, L., Saunders, P., & Benham, A. (2007). A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells. Molecular Biology of the Cell, 18(8), 2795-2804. https://doi.org/10.1091/mbc.e07-02-0147

Journal Article Type	Article
Publication Date	Aug 1, 2007
Deposit Date	Oct 1, 2008
Publicly Available Date	Oct 1, 2008
Journal	Molecular biology of the cell
Publisher	American Society for Cell Biology
Peer Reviewed	Peer Reviewed
Volume	18
Issue	8
Pages	2795-2804
DOI	https://doi.org/10.1091/mbc.e07-02-0147