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Changes in the tobacco leaf apoplast proteome in response to salt stress

Dani, V.; Simon, W.; Duranti, M.; Croy, R.R.D.

Authors

V. Dani

W. Simon

M. Duranti

R.R.D. Croy



Abstract

The apoplast of plant cells is a dynamic compartment involved in many processes, including maintenance of tissue shape, development, nutrition, signalling, detoxification and defence. In this work we used Nicotiana tabacum plants as a model to investigate changes in the soluble apoplast composition induced in response to salt stress. Apoplastic fluid was extracted from leaves of control plants and plants exposed to salt stress, using a vacuum infiltration procedure. Two-dimension electrophoretic analyses revealed about 150 polypeptide spots in the pH range of 3.0 to 10.0, in independent protein extracts, with a high level of reproducibility between the two sample sets. Quantitative evaluation and statistical analyses of the resolved spots in treated and untreated samples revealed 20 polypeptides whose abundance changed in response to salt stress. Mass spectroscopic peptide separation and sequencing was used to identify polypeptides affected by salt stress. While the levels of some proteins were reduced by salt-treatment, an enhanced accumulation of protein species known to be induced by biotic and abiotic stresses was observed. In particular, two chitinases and a germin-like protein increased significantly and two lipid transfer proteins were expressed entirely de novo. Some apoplastic polypeptides, involved in cell wall modifications during plant development, remained largely unchanged. The significance of these components is discussed in the context of stress responses in plants.

Citation

Dani, V., Simon, W., Duranti, M., & Croy, R. (2005). Changes in the tobacco leaf apoplast proteome in response to salt stress. Proteomics, 5(3), 737-745. https://doi.org/10.1002/pmic.200401119

Journal Article Type Article
Publication Date Feb 1, 2005
Deposit Date May 21, 2007
Journal Proteomics
Print ISSN 1615-9853
Electronic ISSN 1615-9861
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 5
Issue 3
Pages 737-745
DOI https://doi.org/10.1002/pmic.200401119
Keywords Apoplast proteins, Salt stress, Tobacco leaf, Two-dimensional gel electrophoresis, Vacuum infiltration.