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Nuclear membrane disassembly and rupture.

Cotter, L. and Allen, T. D. and Kiseleva, E. and Goldberg, M. W. (2007) 'Nuclear membrane disassembly and rupture.', Journal of molecular biology., 369 (3). pp. 683-695.

Abstract

The nuclear envelope consists of two membranes traversed by nuclear pore complexes. The outer membrane is continuous with the endoplasmic reticulum. At mitosis nuclear pore complexes are dismantled and membranes disperse. The mechanism of dispersal is controversial: one view is that membranes feed into the endoplasmic reticulum, another is that they vesiculate. Using Xenopus egg extracts, nuclei have been assembled and then induced to breakdown by addition of metaphase extract. Field emission scanning electron microscopy was used to study disassembly. Strikingly, endoplasmic reticulum-like membrane tubules form from the nuclear surface after the addition of metaphase extracts, but vesicles were also observed. Microtubule inhibitors slowed but did not prevent membrane removal, whereas Brefeldin A, which inhibits vesicle formation, stops membrane disassembly, suggesting that vesiculation is necessary. Structures that looked like coated buds were observed and buds were labelled for β-COP. We show that nuclear pore complexes are dismantled and the pore closed prior to membrane rupturing, suggesting that rupturing is an active process rather than a result of enlargement of nuclear pores.

Item Type:Article
Keywords:Nuclear envelope, Pore complex, Disassembly.
Full text:PDF - Accepted Version (1484Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1016/j.jmb.2007.03.051
Record Created:12 Feb 2009
Last Modified:02 Sep 2011 15:41

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