Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane.

Salpingidou, G. and Smertenko, A. and Hausmanowa-Petrucewicz, I. and Hussey, P. J. and Hutchison, C. J. (2007) 'A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane.', Journal of cell biology., 178 (6). pp. 897-904.

Abstract

The type II inner nuclear membrane protein emerin is a component of the LINC complex that connects the nuclear lamina to the actin cytoskeleton. In emerin-null or -deficient human dermal fibroblasts we find that the centrosome is detached from the nucleus. Moreover, following siRNA knockdown of emerin in wild-type fibroblasts, the centrosome also becomes detached from the nucleus. We show that emerin interacts with tubulin, and that nocadozole-treated wild-type cells phenocopy the detached centrosome characteristic of emerin-null/deficient cells. We also find that a significant fraction of emerin is located at the outer nuclear membrane and peripheral ER, where it interacts directly with the centrosome. Our data provide the first evidence in mammalian cells as to the nature of the linkage of the centrosome, and therefore the tubulin cytoskeleton, with the outer nuclear membrane.

Item Type:Article
Full text:PDF - Published Version (785Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1083/jcb.200702026
Record Created:29 Aug 2008
Last Modified:08 Sep 2011 09:43

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitterExport: EndNote, Zotero | BibTex
Usage statisticsLook up in GoogleScholar | Find in a UK Library