Skip to main content

Research Repository

Advanced Search

A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane

Salpingidou, G; Smertenko, A; Hausmanowa-Petrucewicz, I; Hussey, PJ; Hutchison, CJ

A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane Thumbnail


Authors

G Salpingidou

A Smertenko

I Hausmanowa-Petrucewicz

PJ Hussey

CJ Hutchison



Abstract

The type II inner nuclear membrane protein emerin is a component of the LINC complex that connects the nuclear lamina to the actin cytoskeleton. In emerin-null or -deficient human dermal fibroblasts we find that the centrosome is detached from the nucleus. Moreover, following siRNA knockdown of emerin in wild-type fibroblasts, the centrosome also becomes detached from the nucleus. We show that emerin interacts with tubulin, and that nocadozole-treated wild-type cells phenocopy the detached centrosome characteristic of emerin-null/deficient cells. We also find that a significant fraction of emerin is located at the outer nuclear membrane and peripheral ER, where it interacts directly with the centrosome. Our data provide the first evidence in mammalian cells as to the nature of the linkage of the centrosome, and therefore the tubulin cytoskeleton, with the outer nuclear membrane.

Citation

Salpingidou, G., Smertenko, A., Hausmanowa-Petrucewicz, I., Hussey, P., & Hutchison, C. (2007). A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane. Journal of Cell Biology, 178(6), 897-904. https://doi.org/10.1083/jcb.200702026

Journal Article Type Article
Publication Date Sep 1, 2007
Deposit Date Aug 29, 2008
Publicly Available Date Aug 29, 2008
Journal Journal of Cell Biology
Print ISSN 0021-9525
Electronic ISSN 1540-8140
Publisher Rockefeller University Press
Peer Reviewed Peer Reviewed
Volume 178
Issue 6
Pages 897-904
DOI https://doi.org/10.1083/jcb.200702026

Files





You might also like



Downloadable Citations