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FGF-2 release from the lens capsule by MMP-2 maintains lens epithelial cell viability.

Tholozan, F. M. and Gribbon, C. and Li, Z. and Goldberg, M. W. and Prescott, A. R. and McKie, N. and Quinlan, R. A. (2007) 'FGF-2 release from the lens capsule by MMP-2 maintains lens epithelial cell viability.', Molecular biology of the cell., 18 (11). pp. 4222-4231.

Abstract

The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.

Item Type:Article
Full text:PDF - Published Version (476Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1091/mbc.E06-05-0416
Publisher statement:© 2007 by The American Society for Cell Biology
Record Created:01 Oct 2008
Last Modified:08 Sep 2011 11:02

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