FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Tholozan, F.M.; Gribbon, C.; Li, Z.; Goldberg, M.W.; Prescott, A.R.; McKie, N.; Quinlan, R.A.

doi:10.1091/mbc.e06-05-0416

FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Tholozan, F.M.; Gribbon, C.; Li, Z.; Goldberg, M.W.; Prescott, A.R.; McKie, N.; Quinlan, R.A.

Authors

F.M. Tholozan

C. Gribbon

Z. Li

Professor Martin Goldberg m.w.goldberg@durham.ac.uk
Professor

A.R. Prescott

N. McKie

Professor Roy Quinlan r.a.quinlan@durham.ac.uk
Emeritus Professor

Abstract

The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.

Citation

Tholozan, F., Gribbon, C., Li, Z., Goldberg, M., Prescott, A., McKie, N., & Quinlan, R. (2007). FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability. Molecular Biology of the Cell, 18(11), 4222-4231. https://doi.org/10.1091/mbc.e06-05-0416

Journal Article Type	Article
Publication Date	Aug 1, 2007
Deposit Date	Oct 1, 2008
Publicly Available Date	Oct 1, 2008
Journal	Molecular biology of the cell
Publisher	American Society for Cell Biology
Peer Reviewed	Peer Reviewed
Volume	18
Issue	11
Pages	4222-4231
DOI	https://doi.org/10.1091/mbc.e06-05-0416
Publisher URL	http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17699594