Padmakumar, V. C. and Libotte, T. and Lu, W. S. and Zaim, H. and Abraham, S. and Noegel, A. A. and Gotzmann, J. and Foisner, R. and Karakesisoglou, I. (2005) 'The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope.', Journal of cell science., 118 (15). pp. 3419-3430.
Nesprins form a novel class of nuclear envelope-anchored spectrin-repeat proteins. We show that a direct association of their highly conserved C-terminal luminal domain with the inner nuclear membrane protein Sun1 mediates their nuclear envelope localisation. In Nesprin-1 and Nesprin-2 the conserved C-terminal amino acids PPPX are essential for the interaction with a C-terminal region in Sun1. In fact, Sun1 is required for the proper nuclear envelope localisation of Nesprin-2 as shown using dominant-negative mutants and by knockdown of Sun1 expression. Sun1 itself does not require functional A-type lamins for its localisation at the inner nuclear membrane in mammalian cells. Our findings propose a conserved nuclear anchorage mechanism between Caenorhabditis elegans and mammals and suggest a model in which Sun1 serves as a `structural bridge' connecting the nuclear interior with the actin cytoskeleton.
|Keywords:||Emerin, Enaptin, Lamin A/C, NUANCE, SUN domain, Syne.|
|Full text:||Full text not available from this repository.|
|Publisher Web site:||http://dx.doi.org/10.1242/jcs.02471|
|Record Created:||17 Jan 2008|
|Last Modified:||08 Apr 2009 16:36|
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