Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules

Williamson, D.; Cann, M.J.; Hodgson, D.R.W.

doi:10.1039/b712066d

Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules

Williamson, D.; Cann, M.J.; Hodgson, D.R.W.

Authors

D. Williamson

Professor Martin Cann m.j.cann@durham.ac.uk
Professor

Professor David Hodgson d.r.w.hodgson@durham.ac.uk
Professor

Abstract

5-Amino-5-deoxyguanosine-5-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5-H2N-RNA that can be conjugated to activated esters.

Citation

Williamson, D., Cann, M., & Hodgson, D. (2007). Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules. Chemical Communications, 2007(47), 5096-5098. https://doi.org/10.1039/b712066d

Journal Article Type	Article
Acceptance Date	Sep 19, 2007
Online Publication Date	Oct 11, 2007
Publication Date	Oct 11, 2007
Deposit Date	Jan 18, 2008
Journal	Chemical Communications
Print ISSN	1359-7345
Electronic ISSN	1364-548X
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	2007
Issue	47
Pages	5096-5098
DOI	https://doi.org/10.1039/b712066d