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Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite

Denny, PW; Morgan, GW; Field, MC; Smith, DF

Authors

GW Morgan

MC Field

DF Smith



Abstract

To investigate the role of clathrin-mediated trafficking during the Leishmania lifecycle, open reading frames encoding clathrin heavy chain and the β-adaptins, major components of the adaptor complexes, have been analysed both in silico and experimentally. The Leishmania genome encodes three β-adaptins, which arose at a time predating speciation of these divergent trypanosomatids. Unlike Trypanosoma brucei, both clathrin heavy chain and β-adaptin1 are constitutively expressed throughout the Leishmania life cycle. Clathrin relocalises in amastigotes relative to promastigotes, consistent with developmental alterations to the morphology of the endo-membrane system.

Citation

Denny, P., Morgan, G., Field, M., & Smith, D. (2005). Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite. Experimental Parasitology, 109(1), 33-37. https://doi.org/10.1016/j.exppara.2004.10.007

Journal Article Type Article
Publication Date Jan 1, 2005
Deposit Date May 11, 2009
Journal Experimental Parasitology
Print ISSN 0014-4894
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 109
Issue 1
Pages 33-37
DOI https://doi.org/10.1016/j.exppara.2004.10.007
Keywords Leishmania, Kinetoplastid, Intra-macrophage parasite, Clathrin, Adaptin, Evolution, Trafficking, Protein complex, Parasitophorous vacuoles, Mediated endocytosis, Trypanosoma-brucei, Macrophages, Amazonensis, Protozoan, Molecules, Mexicana, System.