The Synergistic Action of Melittin and Phospholipase A2 with Lipid Membranes: Development of Linear Dichroism for Membrane-Insertion Kinetics

Damianoglou, Angeliki; Rodger, Alison; Pridmore, Catherine; Dafforn, Timothy R.; Mosely, Jackie A.; Sanderson, John M.; Hicks, Matthew R.

doi:10.2174/0929866511009011351

The Synergistic Action of Melittin and Phospholipase A2 with Lipid Membranes: Development of Linear Dichroism for Membrane-Insertion Kinetics

Damianoglou, Angeliki; Rodger, Alison; Pridmore, Catherine; Dafforn, Timothy R.; Mosely, Jackie A.; Sanderson, John M.; Hicks, Matthew R.

Authors

Angeliki Damianoglou

Alison Rodger

Catherine Pridmore

Timothy R. Dafforn

Jackie A. Mosely

Dr John Sanderson j.m.sanderson@durham.ac.uk
Associate Professor

Matthew R. Hicks

Abstract

Here we present data on the kinetics of insertion of melittin, a peptide from bee venom, into lipid membranes of different composition. Another component of bee venom is the enzyme phospholipase A2 (PLA₂). We have examined the interaction of melittin and PLA₂ with liposomes both separately and combined and demonstrate that they work synergistically to disrupt the membranes. A dramatic difference in the action of melittin and PLA₂ is observed when the composition of the membrane is altered. Temperature also has a large effect on the kinetics of insertion and membrane disruption. We use a combination of techniques to measure liposome size (dynamic light scattering), peptide secondary structure (circular dichroism spectroscopy), peptide orientation relative to the membrane (linear dichroism spectroscopy) and enzymatic digestion of the lipids (mass spectrometry).

Citation

Damianoglou, A., Rodger, A., Pridmore, C., Dafforn, T. R., Mosely, J. A., Sanderson, J. M., & Hicks, M. R. (2010). The Synergistic Action of Melittin and Phospholipase A2 with Lipid Membranes: Development of Linear Dichroism for Membrane-Insertion Kinetics. Protein and Peptide Letters, 17(11), 1351-1362. https://doi.org/10.2174/0929866511009011351

Journal Article Type	Article
Publication Date	Nov 1, 2010
Deposit Date	Feb 9, 2011
Publicly Available Date	Jan 3, 2012
Journal	Protein and Peptide Letters
Print ISSN	0929-8665
Publisher	Bentham Science Publishers
Peer Reviewed	Not Peer Reviewed
Volume	17
Issue	11
Pages	1351-1362
DOI	https://doi.org/10.2174/0929866511009011351
Keywords	Amphipathic, Peptide, Antimicrobial, Membrane protein, Linear dichroism, Liposome, Dynamic light scattering, Membrane, Linear, Dynamic, (PLA2), (mass spectrometry), Crystallography, NMR, (LD), Melittin, (DOPC), DOPG, (POPC), DMPC, (TLC), (OCD), (PDPC).