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Influence of lipids on the interfacial disposition of respiratory syncytical virus matrix protein.

McPhee, Helen K. and Carlisle, Jennifer L. and Beeby, Andrew and Money, Victoria A. and Watson, Scott M. D. and Yeo, R. Paul and Sanderson, John M. (2011) 'Influence of lipids on the interfacial disposition of respiratory syncytical virus matrix protein.', Langmuir., 27 (1). pp. 304-311.

Abstract

The propensity of a matrix protein from an enveloped virus of the Mononegavirales family to associate with lipids representative of the viral envelope has been determined using label-free methods, including tensiometry and Brewster angle microscopy on lipid films at the air−water interface and atomic force microscopy on monolayers transferred to OTS-treated silicon wafers. This has enabled factors that influence the disposition of the protein with respect to the lipid interface to be characterized. In the absence of sphingomyelin, respiratory syncytial virus matrix protein penetrates monolayers composed of mixtures of phosphocholines with phosphoethanolamines or cholesterol at the air−water interface. In ternary mixtures composed of sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol, the protein exhibits two separate behaviors: (1) peripheral association with the surface of sphingomyelin-rich domains and (2) penetration of sphingomyelin-poor domains. Prolonged incubation of the protein with mixtures of phosphocholines and phosphoethanolamines leads to the formation of helical protein assemblies of uniform diameter that demonstrate an inherent propensity of the protein to assemble into a filamentous form.

Item Type:Article
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1021/la104041n
Record Created:21 Jun 2011 14:35
Last Modified:08 Jun 2012 16:41

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