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The Arabidopsis phi class glutathione transferase AtGSTF2: binding and regulation by biologically active heterocyclic ligands

Dixon, D.P.; Sellars, J.D.; Edwards, R.

The Arabidopsis phi class glutathione transferase AtGSTF2: binding and regulation by biologically active heterocyclic ligands Thumbnail


Authors

D.P. Dixon

J.D. Sellars

R. Edwards



Abstract

The plant-specific phi class of glutathione transferases (GSTFs) are often highly stress-inducible and expressed in a tissue-specific manner, suggestive of them having important protective roles. To date, these functions remain largely unknown, although activities associated with the binding and transport of reactive metabolites have been proposed. Using a sensitive and selective binding screen, we have probed the Arabidopsis thaliana GSTFs for natural product ligands from bacteria and plants. Uniquely, when over-expressed in bacteria, family members GSTF2 and GSTF3 bound a series of heterocyclic compounds including lumichrome, harmane, norharmane and indole-3-aldehyde. When screened against total metabolite extracts from A. thaliana, GSTF2 also selectively bound the indole-derived phytoalexin camalexin as well as the flavonol quercetin-3-O-rhamnoside. In each case, isothermal titration calorimetry revealed high affinity binding (typically Kd < 1 µM), which was enhanced in the presence of glutathione and by the other heterocyclic ligands. With GSTF2, these secondary ligand associations resulted in an allosteric enhancement in glutathione conjugating activity. Together with the known stress responsiveness of GSTF2 and its association with membrane vesicles, these results are suggestive of roles in regulating the binding and transport of defence-related compounds in planta.

Citation

Dixon, D., Sellars, J., & Edwards, R. (2011). The Arabidopsis phi class glutathione transferase AtGSTF2: binding and regulation by biologically active heterocyclic ligands. Biochemical Journal, 438(1), 63-70. https://doi.org/10.1042/bj20101884

Journal Article Type Article
Publication Date Aug 1, 2011
Deposit Date Jun 7, 2011
Publicly Available Date Mar 28, 2024
Journal Biochemical Journal
Print ISSN 0264-6021
Publisher Portland Press
Peer Reviewed Peer Reviewed
Volume 438
Issue 1
Pages 63-70
DOI https://doi.org/10.1042/bj20101884
Keywords Flavonoid, Indole, Ligandin, Phi class glutathione transferase 2 (GSTF2), Phytoalexin, Plant defence.

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Copyright Statement
The Version of Record (VoR) is available at www.biochemj.org





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