Cookies

We use cookies to ensure that we give you the best experience on our website. You can change your cookie settings at any time. Otherwise, we'll assume you're OK to continue.


Durham Research Online
You are in:

Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes.

New, E.J. and Congreve, A. and Parker, D. (2010) 'Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes.', Chemical science., 1 (1). pp. 111-118.

Abstract

A series of experiments has been undertaken in order to gain a greater understanding of the cellular uptake and localisation behaviour of emissive lanthanide complexes as cellular stains or probes. Out of a large number of structurally related complexes characterised recently, a set of seven representative examples has been examined in detail, containing either tetraazatriphenylene or azaxanthone-based sensitising chromophores. Intracellular localisation profiles and cellular uptake and egress behaviour have been studied by microscopy and flow cytometry. Typically, the maximum intracellular concentration was of the order of 0.4 mM, or about 109 complexes per cell. The complexes studied were generally not toxic and did not perturb the mitochondrial membrane potential. A common uptake mechanism of macropinocytosis has been identified. A generalisation of trends in behaviour, and structure–activity relationships is presented, and the implications for future probe design discussed.

Item Type:Article
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1039/c0sc00105h
Record Created:10 Jan 2012 11:20
Last Modified:17 Jul 2012 16:36

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitterExport: EndNote, Zotero | BibTex
Usage statisticsLook up in GoogleScholar | Find in a UK Library