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Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope.

Malik, P. and Korfali, N. and Srsen, V. and Lazou, V. and Batrakou, D.G. and Zuleger, N. and Kavanagh, D.M. and Wilkie, G.S. and Goldberg, M.W. and Schirmer, E.C. (2010) 'Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope.', Cellular and molecular life sciences., 67 (8). pp. 1353-1369.


Nuclear envelope complexity is expanding with respect to identification of protein components. Here we test the validity of proteomics results that identified 67 novel predicted nuclear envelope transmembrane proteins (NETs) from liver by directly comparing 30 as tagged fusions using targeting assays. This confirmed 21 as NETs, but 4 only targeted in certain cell types, underscoring the complexity of interactions that tether NETs to the nuclear envelope. Four NETs accumulated at the nuclear rim in normal fibroblasts but not in fibroblasts lacking lamin A, suggesting involvement of lamin A in tethering them in the nucleus. However, intriguingly, for the NETs tested alternative mechanisms for nuclear envelope retention could be found in Jurkat cells that normally lack lamin A. This study expands by a factor of three the number of liver NETs analyzed, bringing the total confirmed to 31, and shows that several have multiple mechanisms for nuclear envelope retention.

Item Type:Article
Keywords:Inner nuclear membrane, Outer nuclear membrane, Nuclear lamina, Integral membrane protein, Cell-type specificity.
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Publisher statement:This article is published under a Creative Commons Attribution (CC-BY) license
Record Created:01 Jun 2012 10:05
Last Modified:12 Jun 2012 10:37

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