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A cytosolic Ni(II) sensor in cyanobacterium : nickel-detection follows nickel-affinity across four families of metal-sensors.

Foster, A.W. and Patterson, C.J. and Pernil, R. and Hess, C.R. and Robinson, N.J. (2012) 'A cytosolic Ni(II) sensor in cyanobacterium : nickel-detection follows nickel-affinity across four families of metal-sensors.', Journal of biological chemistry., 287 (15). pp. 12142-12151.

Abstract

Efflux of surplus Ni(II) across the outer and inner membranes of Synechocystis PCC 6803 is mediated by the Nrs system under the control of a sensor of periplasmic Ni(II), NrsS. Here, we show that the product of ORF sll0176, which encodes a CsoR/RcnR-like protein now designated InrS (for internal nickel-responsive sensor), represses nrsD (NrsD is deduced to efflux Ni(II) across the inner membrane) from a cryptic promoter between the final two ORFs in the nrs operon. Transcripts initiated from the newly identified nrsD promoter accumulate in response to nickel or cobalt but not copper, and recombinant InrS forms specific, Ni(II)-inhibited complexes with the nrsD promoter region. Metal-dependent difference spectra of Ni(II)- and Cu(I)-InrS are similar to Cu(I)-sensing CsoR and dissimilar to Ni(II)/Co(II)-sensing RcnR, consistent with factors beyond the primary coordination sphere switching metal selectivity. Competition with chelators mag-fura-2, nitrilotriacetic acid, EDTA, and EGTA estimate KD Ni(II) for the tightest site of InrS as 2.05 (±1.5) × 10−14 m, and weaker KD Ni(II) for the cells' metal sensors of other types: Zn(II) co-repressor Zur, Co(II) activator CoaR, and Zn(II) derepressor ZiaR. Ni(II) transfer to InrS occurs upon addition to Ni(II) forms of each other sensor. InrS binds Ni(II) sufficiently tightly to derepress Ni(II) export at concentrations below KD Ni(II) of the other sensors.

Item Type:Article
Keywords:Hydrogenase, Metal Homeostasis, Metalloproteins, Metals, Nickel, ArsR/SmtB Family, CsoR/RcnR Family, Fur Family, MerR Family, Metal Specificity.
Full text:PDF - Published Version (1668Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1074/jbc.M111.338301
Publisher statement:Full-text available under a Creative Commons Attribution Non-Commercial License.
Record Created:01 Jun 2012 12:35
Last Modified:06 Jun 2012 10:04

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