AW Foster
A cytosolic Ni(II) sensor in cyanobacterium: Nickel-detection follows nickel-affinity across four families of metal-sensors
Foster, AW; Patterson, CJ; Pernil, R; Hess, CR; Robinson, NJ
Authors
Abstract
Efflux of surplus Ni(II) across the outer and inner membranes of Synechocystis PCC 6803 is mediated by the Nrs system under the control of a sensor of periplasmic Ni(II), NrsS. Here, we show that the product of ORF sll0176, which encodes a CsoR/RcnR-like protein now designated InrS (for internal nickel-responsive sensor), represses nrsD (NrsD is deduced to efflux Ni(II) across the inner membrane) from a cryptic promoter between the final two ORFs in the nrs operon. Transcripts initiated from the newly identified nrsD promoter accumulate in response to nickel or cobalt but not copper, and recombinant InrS forms specific, Ni(II)-inhibited complexes with the nrsD promoter region. Metal-dependent difference spectra of Ni(II)- and Cu(I)-InrS are similar to Cu(I)-sensing CsoR and dissimilar to Ni(II)/Co(II)-sensing RcnR, consistent with factors beyond the primary coordination sphere switching metal selectivity. Competition with chelators mag-fura-2, nitrilotriacetic acid, EDTA, and EGTA estimate KD Ni(II) for the tightest site of InrS as 2.05 (±1.5) × 10−14 m, and weaker KD Ni(II) for the cells' metal sensors of other types: Zn(II) co-repressor Zur, Co(II) activator CoaR, and Zn(II) derepressor ZiaR. Ni(II) transfer to InrS occurs upon addition to Ni(II) forms of each other sensor. InrS binds Ni(II) sufficiently tightly to derepress Ni(II) export at concentrations below KD Ni(II) of the other sensors.
Citation
Foster, A., Patterson, C., Pernil, R., Hess, C., & Robinson, N. (2012). A cytosolic Ni(II) sensor in cyanobacterium: Nickel-detection follows nickel-affinity across four families of metal-sensors. Journal of Biological Chemistry, 287(15), 12142-12151. https://doi.org/10.1074/jbc.m111.338301
Journal Article Type | Article |
---|---|
Publication Date | Apr 1, 2012 |
Deposit Date | Apr 2, 2012 |
Publicly Available Date | Mar 29, 2024 |
Journal | Journal of Biological Chemistry |
Print ISSN | 0021-9258 |
Electronic ISSN | 1083-351X |
Publisher | American Society for Biochemistry and Molecular Biology |
Peer Reviewed | Peer Reviewed |
Volume | 287 |
Issue | 15 |
Pages | 12142-12151 |
DOI | https://doi.org/10.1074/jbc.m111.338301 |
Keywords | Hydrogenase, Metal Homeostasis, Metalloproteins, Metals, Nickel, ArsR/SmtB Family, CsoR/RcnR Family, Fur Family, MerR Family, Metal Specificity. |
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