H Okamoto
The alpha-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana
Okamoto, H; Gobel, C; Capper, RG; Saunders, N; Feussner, I; Knight, MR
Authors
C Gobel
RG Capper
N Saunders
I Feussner
MR Knight
Abstract
Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the Gα subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated that the Gα subunit may play a role in response to jasmonic acid (JA). Consistent with this, Gα mutants showed a reduced response to JA in inhibition of chlorophyll accumulation and root growth, whilst Gα gain-of-function plants overexpressing Gα showed the opposite phenotype. The levels of JA and related compounds were unaffected in the gpa1-1 mutant, as was autoregulation of the Allene Oxide Synthase (AOS) gene that encodes a key enzyme for JA biosynthesis. In contrast, further analyses using Gα loss- and gain-of-function Arabidopsis lines indicated that Gα positively modulates the expression of the Vegetative Storage Protein (VSP) gene. This indicates that the Gα subunit regulates a subset of JA-regulated genes defining a branch point in this signalling pathway in Arabidopsis. Further analysis of the impact of Gα loss of function upon the JA-regulated transcriptome using Arabidopsis full genome arrays indicated that up to 29% of genes that are >2-fold regulated by JA in the wild type are misregulated in the Gα mutant. This supports the observation that a significant proportion of, but not all, JA-regulated gene expression is mediated by Gα.
Citation
Okamoto, H., Gobel, C., Capper, R., Saunders, N., Feussner, I., & Knight, M. (2009). The alpha-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana. Journal of Experimental Botany, 60(7), 1991-2003. https://doi.org/10.1093/jxb/erp060
Journal Article Type | Article |
---|---|
Publication Date | May 1, 2009 |
Deposit Date | Jun 6, 2012 |
Publicly Available Date | Jun 8, 2012 |
Journal | Journal of Experimental Botany |
Print ISSN | 0022-0957 |
Electronic ISSN | 1460-2431 |
Publisher | Oxford University Press |
Peer Reviewed | Peer Reviewed |
Volume | 60 |
Issue | 7 |
Pages | 1991-2003 |
DOI | https://doi.org/10.1093/jxb/erp060 |
Keywords | AOS, Arabidopsis, Heterotrimeric G-protein, G-protein alpha-subunit (GPA1), Jasmonic acid, PDF1.2, VSP, Abscisic-acid, Methyl jasmonate, Beta-subunit, Transcription factor, Cell-proliferation, Disease resistance, Seed-germination, Coupled receptor. |
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