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Multidepth, multiparticle tracking for active microrheology using a smart camera.

Silburn, Scott A. and Saunter, Christopher D. and Girkin, John M. and Love, Gordon D. (2011) 'Multidepth, multiparticle tracking for active microrheology using a smart camera.', Review of scientific instruments., 82 (3). 033712.

Abstract

The quantitative measurement of particle motion in optical tweezers is an important tool in the study of microrheology and can be used in a variety of scientific and industrial applications. Active microheology, in which the response of optically trapped particles to external driving forces is measured, is particularly useful in probing nonlinear viscoelastic behavior in complex fluids. Currently such experiments typically require independent measurements of the driving force and the trapped particle's response to be carefully synchronized, and therefore the experiments normally require analog equipment. In this paper we describe both a specialized camera and an imaging technique which make high-speed video microscopy a suitable tool for performing such measurements, without the need for separate measurement systems and synchronization. The use of a high-speed tracking camera based on a field programmable gate array to simultaneously track multiple particles is reported. By using this camera to simultaneously track one microsphere fixed to the wall of a driven sample chamber and another held in an optical trap, we demonstrate simultaneous optical measurement of the driving motion and the trapped probe particle response using a single instrument. Our technique is verified experimentally by active viscosity measurements on water–ethylene glycol mixtures using a phase-shift technique.

Item Type:Article
Full text:PDF - Published Version (274Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1063/1.3567801
Publisher statement:© 2011 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Silburn, Scott A. and Saunter, Christopher D. and Girkin, John M. and Love, Gordon D. (2011) 'Multidepth, multiparticle tracking for active microrheology using a smart camera.', Review of scientific instruments., 82 (3). 033712 and may be found at http://dx.doi.org/10.1063/1.3567801
Record Created:12 Sep 2012 10:50
Last Modified:10 Jan 2013 16:35

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