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5′-Deoxy-5′-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs.

Skipsey, M. and Hack, G. and Hooper, T. A. and Shankey, M. C. and Conway, L. P. and Schröder, M. and Hodgson, D. R. W. (2013) '5′-Deoxy-5′-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs.', Nucleosides, nucleotides, and nucleic acids., 32 (12). pp. 670-681.

Abstract

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.

Item Type:Article
Keywords:T7 RNA polymerase initiator, Fluorophore-labeled RNA.
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Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1080/15257770.2013.851393
Publisher statement:© Skipsey et al. Published with License by Taylor & Francis This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. Permission is granted subject to the terms of the License under which the work was published. Please check the License conditions for the work which you wish to reuse. Full and appropriate attribution must be given. This permission does not cover any third party copyrighted material which may appear in the work requested.
Date accepted:No date available
Date deposited:16 July 2014
Date of first online publication:December 2013
Date first made open access:No date available

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