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A gradient of matrix-bound FGF-2 and perlecan is available to lens epithelial cells.

Wu, W. and Tholozan, F. M. and Goldberg, M. W. and Bowen, L. and Wu, J.J. and Quinlan, R. A. (2014) 'A gradient of matrix-bound FGF-2 and perlecan is available to lens epithelial cells.', Experimental eye research., 120 . pp. 10-14.

Abstract

Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the presentation of FGF-2 to the lens epithelial cells involves the lens capsule as a source of matrix-bound FGF-2. Here we used immunogold labelling to measure the matrix-bound FGF-2 gradient on the inner surface of the lens capsule in flat-mounted preparations to visualize the FGF-2 available to lens epithelial cells. We also correlated FGF-2 levels with levels of its matrix-binding partner perlecan, a heparan sulphate proteoglycan (HSPG) and found the levels of both to be highest at the lens equator. These also coincided with increased levels of phosphorylated extracellular signal-regulated kinase 1 and 2 (pERK1/2) in lens epithelial cells that localised to condensed chromosomes of epithelial cells that were Ki-67 positive. The gradient of matrix-bound FGF-2 (anterior pole: 3.7 ± 1.3 particles/μm2; equator: 8.2 ± 1.9 particles/μm2; posterior pole: 4 ± 0.9 particles/μm2) and perlecan (anterior pole: 2.1 ± 0.4 particles/μm2; equator: 5 ± 2 particles/μm2; posterior pole: 1.9 ± 0.7 particles/μm2) available at the inner lens capsule surface was measured for the bovine lens. These data support the anteroposterior gradient hypothesis and provide the first measurement of the gradient for an important morphogen and its HSPG partner, perlecan, at the epithelial cell-lens capsule interface.

Item Type:Article
Keywords:Lens capsule, FGF-2, Perlecan, ERK1/2.
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Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1016/j.exer.2013.12.004
Publisher statement:This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
Date accepted:30 November 2014
Date deposited:06 May 2014
Date of first online publication:March 2014
Date first made open access:No date available

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