3D adaptive optics in a light sheet microscope

Bourgenot, Cyril; Saunter, Christopher D.; Taylor, Jonathan M.; Girkin, John M.; Love, Gordon D.

doi:10.1364/oe.20.013252

3D adaptive optics in a light sheet microscope

Bourgenot, Cyril; Saunter, Christopher D.; Taylor, Jonathan M.; Girkin, John M.; Love, Gordon D.

Authors

Cyril Bourgenot cyril.bourgenot@durham.ac.uk
Associate Professor

Christopher D. Saunter

Jonathan M. Taylor

Professor John Girkin j.m.girkin@durham.ac.uk
Professor

Gordon D. Love

Abstract

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.

Citation

Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), 13252-13261. https://doi.org/10.1364/oe.20.013252

Journal Article Type	Article
Publication Date	Jun 1, 2012
Deposit Date	May 22, 2014
Publicly Available Date	Jul 29, 2014
Journal	Optics Express
Publisher	Optica
Peer Reviewed	Peer Reviewed
Volume	20
Issue	12
Article Number	13252-13261
Pages	13252-13261
DOI	https://doi.org/10.1364/oe.20.013252

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Copyright Statement
This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.20.013252. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.