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3D adaptive optics in a light sheet microscope.

Bourgenot, Cyril and Saunter, Christopher D. and Taylor, Jonathan M. and Girkin, John M. and Love, Gordon D. (2012) '3D adaptive optics in a light sheet microscope.', Optics express., 20 (12). pp. 13252-13261.


We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.

Item Type:Article
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Publisher statement:This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
Date accepted:No date available
Date deposited:29 July 2014
Date of first online publication:June 2012
Date first made open access:No date available

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