Time-Resolved Photodetachment Anisotropy: Gas-Phase Rotational and Vibrational Dynamics of the Fluorescein Anion

Horke, D.A.; Chatterley, A.S.; Bull, J.N.; Verlet, J.R.R.

doi:10.1021/jz5022526

Time-Resolved Photodetachment Anisotropy: Gas-Phase Rotational and Vibrational Dynamics of the Fluorescein Anion

Horke, D.A.; Chatterley, A.S.; Bull, J.N.; Verlet, J.R.R.

Authors

D.A. Horke

A.S. Chatterley

J.N. Bull

Professor Jan Verlet j.r.r.verlet@durham.ac.uk
Professor

Abstract

The photoelectron signal of the singly deprotonated fluorescein anion is found to be highly dependent on the relative polarization between pump and probe pulses, and time-resolved photodetachment anisotropy (TR-PA) is developed as a probe of the rotational dynamics of the chromophore. The total photoelectron signal shows both rotational and vibrational wavepacket dynamics, and we demonstrate how TR-PA can readily disentangle these dynamical processes. TR-PA in fluorescein presents specific opportunities for its development as a probe for rotational dynamics in large biomolecules as fluorescein derivatives are commonly incorporated in complex biomolecules and have been used extensively in time-resolved fluorescence anisotropy measurements, to which TR-PA is a gas-phase analogue.

Citation

Horke, D., Chatterley, A., Bull, J., & Verlet, J. (2015). Time-Resolved Photodetachment Anisotropy: Gas-Phase Rotational and Vibrational Dynamics of the Fluorescein Anion. Journal of Physical Chemistry Letters, 6(1), 189-194. https://doi.org/10.1021/jz5022526

Journal Article Type	Article
Publication Date	Jan 2, 2015
Deposit Date	Feb 18, 2015
Publicly Available Date	Feb 25, 2015
Journal	Journal of Physical Chemistry Letters
Publisher	American Chemical Society
Peer Reviewed	Peer Reviewed
Volume	6
Issue	1
Pages	189-194
DOI	https://doi.org/10.1021/jz5022526
Keywords	Gas-phase anions, Ultrafast dynamics, Electron detachment, Anisotropy, Photoelectron spectroscopy, Fluorescein, Wavepacket dynamics, Rotational anisotropy.

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Copyright Statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry Letters, copyright © 2014 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/jz5022526.