He, Q. and McLellan, H. and Boevink, P.C. and Sadanandom, A. and Xie, C. and Birch, P.R. and Tian, Z. (2015) 'U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.', Journal of experimental botany., 66 (11). pp. 3189-3199.
Abstract
Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17V314I,V316I mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17V314I,V316I mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways.
| Item Type: | Article |
|---|---|
| Keywords: | Disease resistance, Effector-triggered immunity, Hypersensitive response, Oomycete, Plant defence Ubiquitination |
| Full text: | (VoR) Version of Record Available under License - Creative Commons Attribution. Download PDF (3094Kb) |
| Status: | Peer-reviewed |
| Publisher Web site: | http://dx.doi.org/10.1093/jxb/erv128 |
| Publisher statement: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Date accepted: | No date available |
| Date deposited: | 09 October 2015 |
| Date of first online publication: | June 2015 |
| Date first made open access: | No date available |
Save or Share this output
| Export: | |
| Look up in GoogleScholar |
