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Base-specific ionization of deprotonated nucleotides by resonance enhanced two-photon detachment.

Chatterley, A. S. and Johns, A. S. and Stavros, V. G. and Verlet, J. R. R. (2013) 'Base-specific ionization of deprotonated nucleotides by resonance enhanced two-photon detachment.', Journal of physical chemistry A., 117 (25). pp. 5299-5305.


The intrinsic ionization energy of a base in DNA plays a critical role in determining the energies at which damage mechanisms may emerge. Here, a two-photon resonance-enhanced ionization scheme is presented that utilizes the 1ππ* transition, localized on the DNA base, to elucidate the base-specific ionization in a deprotonated nucleotide. In contrast to previous reports, the scheme is insensitive to competing ionization channels arising from the sugar–phosphate backbone. Using this approach, we demonstrate that for all bases except guanine, the lowest electron detachment energy corresponds to detachment from the sugar–phosphate backbone and allows us to determine the lowest adiabatic ionization energy for the other three bases for the first time in an isolated nucleotide.

Item Type:Article
Full text:(AM) Accepted Manuscript
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Publisher statement:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry A, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
Date accepted:No date available
Date deposited:28 January 2016
Date of first online publication:June 2013
Date first made open access:No date available

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