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PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis.

Ikeuchi, Momoko and Iwase, Akira and Rymen, Bart and Harashima, Hirofumi and Shibata, Michitaro and Ohnuma, Mariko and Breuer, Christian and Morao, Ana Karina and de Lucas, Miguel and De Veylder, Lieven and Goodrich, Justin and Brady, Siobhan M. and Roudier, François and Sugimoto, Keiko (2015) 'PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis.', Nature plants., 1 (7). p. 15089.


Plant somatic cells are generally acknowledged to retain totipotency, the potential to develop into any cell type within an organism. This astonishing plasticity may contribute to a high regenerative capacity on severe damage, but how plants control this potential during normal post-embryonic development remains largely unknown1,2. Here we show that POLYCOMB REPRESSIVE COMPLEX 2 (PRC2), a chromatin regulator that maintains gene repression through histone modification, prevents dedifferentiation of mature somatic cells in Arabidopsis thaliana roots. Loss-of-function mutants in PRC2 subunits initially develop unicellular root hairs indistinguishable from those in wild type but fail to retain the differentiated state, ultimately resulting in the generation of an unorganized cell mass and somatic embryos from a single root hair. Strikingly, mutant root hairs complete the normal endoreduplication programme, increasing their nuclear ploidy, but subsequently reinitiate mitotic division coupled with successive DNA replication. Our data show that the WOUND INDUCED DEDIFFERENTIATION3 (WIND3) and LEAFY COTYLEDON2 (LEC2) genes are among the PRC2 targets involved in this reprogramming, as their ectopic overexpression partly phenocopies the dedifferentiation phenotype of PRC2 mutants. These findings unveil the pivotal role of PRC2-mediated gene repression in preventing unscheduled reprogramming of fully differentiated plant cells.

Item Type:Article
Full text:(AM) Accepted Manuscript
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Date accepted:29 May 2015
Date deposited:18 March 2016
Date of first online publication:July 2015
Date first made open access:No date available

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