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Poor preservation of antibodies in archaeological human bone and dentine.

Kendall, R. and Hendy, J. and Collins, M. J. and Millard, A. R. and Gowland, R. L. (2016) 'Poor preservation of antibodies in archaeological human bone and dentine.', Science and technology of archaeological research., 2 (1). pp. 15-24.

Abstract

The growth of proteomics-based methods in archaeology prompted an investigation of the survival of non-collagenous proteins, specifically immunoglobulin G (IgG), in archaeological human bone and dentine. Over a decade ago reports were published on extracted, immunoreactive archaeological IgG, and the variable yields of IgG molecules detected by Western blots of 1D and 2D SDS-PAGE gels. If IgG can indeed be recovered from archaeological skeletal material, it offers remarkable opportunities for exploring the history of disease - for example in applying functional anti-malarial IgGs to study past patterns of malaria. More recently, the field has seen a move away from immunological approaches and towards the use of shotgun proteomics via mass spectrometry. Using previously published techniques, this study attempted to extract and characterize archaeological IgG proteins. In only one extraction method were immunoglobulin derived peptides identified, and these displayed extensive evidence of degradation. The failure to extract immunoglobulins by all but one method, along with observed patterns of protein degradation, suggests that IgG may be an unsuitable target for detecting disease-associated antigens. This research highlights the importance of revisiting previously ‘successful’ biomolecular methodologies using emerging technologies.

Item Type:Article
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Available under License - Creative Commons Attribution.
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Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1080/20548923.2015.1133117
Publisher statement:© 2016 Taylor & Francis This article is Open Access and is distributed under the terms of the Creative Commons Attribution License 4.0
Date accepted:07 July 2015
Date deposited:16 March 2016
Date of first online publication:25 February 2016
Date first made open access:16 March 2016

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