Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

An initial phase of JNK activation inhibits cell death early in the endoplasmic reticulum stress response.

Brown, M. and Strudwick, N. and Suwara, M. and Sutcliffe, L. K. and Mihai, A. D. and Ali, A. A. and Watson, J. N. and Schröder, M. (2016) 'An initial phase of JNK activation inhibits cell death early in the endoplasmic reticulum stress response.', Journal of cell science., 129 (12). pp. 2317-2328.

Abstract

Accumulation of unfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR). In mammalian cells, UPR signals generated by several ER membrane resident proteins, including the bifunctional protein kinase endoribonuclease IRE1α, control cell survival and the decision to execute apoptosis. Processing of XBP1 mRNA by the RNase domain of IRE1α promotes survival of ER stress, while activation of the mitogen-activated protein kinase JNK by IRE1α late in the ER stress response promotes apoptosis. Here we show that activation of JNK in the ER stress response precedes activation of XBP1. This activation of JNK is dependent on IRE1α and TRAF2 and coincides with JNK-dependent induction of expression of several antiapoptotic genes, including cIAP1, cIAP2, XIAP, and BIRC6. ER-stressed jnk1−/− jnk2−/− mouse embryonic fibroblasts (MEFs) display more pronounced mitochondrial permeability transition and increased caspase 3/7 activity compared to wild type MEFs. Caspase 3/7 activity is also elevated in ER-stressed ciap1−/− ciap2−/−, and xiap−/− MEFs. These observations suggest that JNK-dependent transcriptional induction of several inhibitors of apoptosis contributes to inhibiting apoptosis early in the ER stress response.

Item Type:Article
Full text:(AM) Accepted Manuscript
Download PDF
(3337Kb)
Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1242/jcs.179127
Date accepted:20 April 2016
Date deposited:21 April 2016
Date of first online publication:27 April 2016
Date first made open access:27 April 2017

Save or Share this output

Export:
Export
Look up in GoogleScholar