We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.

Durham Research Online
You are in:

Transcriptional regulation of Arabidopsis Polycomb Repressive Complex 2 coordinates cell type proliferation and differentiation.

de Lucas, Miguel and Pu, Li and Turco, Gina Marie and Gaudinier, Allie and Marao, Ana Karina and Hiroshima, Hirofumi and Kim, Dahae and Ron, Mily and Sugimoto, Keiko and Roudier, Francois M. and Brady, Siobhan M. (2016) 'Transcriptional regulation of Arabidopsis Polycomb Repressive Complex 2 coordinates cell type proliferation and differentiation.', Plant cell., 28 (10). pp. 2616-2631.


Spatiotemporal regulation of transcription is fine-tuned at multiple levels, including chromatin compaction. Polycomb Repressive Complex 2 (PRC2) catalyzes the trimethylation of Histone 3 at lysine 27 (H3K27me3), which is the hallmark of a repressive chromatin state. Multiple PRC2 complexes have been reported in Arabidopsis thaliana to control the expression of genes involved in developmental transitions and maintenance of organ identity. Here, we show that PRC2 member genes display complex spatiotemporal gene expression patterns and function in root meristem and vascular cell proliferation and specification. Furthermore, PRC2 gene expression patterns correspond with vascular and non-vascular tissue-specific H3K27me3-marked genes. This tissue-specific repression via H3K27me3 regulates the balance between cell proliferation and differentiation. Using enhanced yeast-one-hybrid analysis, upstream regulators of the PRC2 member genes are identified, and genetic analysis demonstrates that transcriptional regulation of some PRC2 genes plays an important role in determining PRC2 spatiotemporal activity within a developing organ.

Item Type:Article
Full text:(VoR) Version of Record
Download PDF (Advance online version)
Full text:(VoR) Version of Record
Download PDF (Final published version)
Publisher Web site:
Publisher statement:Copyright American Society of Plant Biologists.
Date accepted:14 September 2016
Date deposited:26 September 2016
Date of first online publication:20 September 2016
Date first made open access:26 September 2016

Save or Share this output

Look up in GoogleScholar