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Durham Research Online
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Advancing age decreases pressure-sensitive modulation of calcium signaling in the endothelium of intact and pressurized arteries.

Wilson, Calum and Saunter, Christopher D. and Girkin, John M. and McCarron, John G. (2017) 'Advancing age decreases pressure-sensitive modulation of calcium signaling in the endothelium of intact and pressurized arteries.', Journal of vascular research., 53 (5-6). pp. 358-369.

Abstract

Aging is the summation of many subtle changes which result in altered cardiovascular function. Impaired endothelial function underlies several of these changes and precipitates plaque development in larger arteries. The endothelium transduces chemical and mechanical signals into changes in the cytoplasmic calcium concentration to control vascular function. However, studying endothelial calcium signaling in larger arteries in a physiological configuration is challenging because of the requirement to focus through the artery wall. Here, pressure- and agonist-sensitive endothelial calcium signaling was studied in pressurized carotid arteries from young (3-month-old) and aged (18-month-old) rats by imaging from within the artery using gradient index fluorescence microendoscopy. Endothelial sensitivity to acetylcholine increased with age. The number of cells exhibiting oscillatory calcium signals and the frequency of oscillations were unchanged with age. However, the latency of calcium responses was significantly increased with age. Acetylcholine-evoked endothelial calcium signals were suppressed by increased intraluminal pressure. However, pressure-dependent inhibition of calcium signaling was substantially reduced with age. While each of these changes will increase endothelial calcium signaling with increasing age, decreases in endothelial pressure sensitivity may manifest as a loss of functionality and responsiveness in aging.

Item Type:Article
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Available under License - Creative Commons Attribution.
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1159/000454811
Publisher statement:© 2017 The Author(s) Published by S. Karger AG, Basel This article is licensed under the Creative Commons Attribution 4.0 International License (CC BY) (http://www.karger.com/Services/ OpenAccessLicense). Usage, derivative works and distribution are permitted provided that proper credit is given to the author and the original publisher.
Date accepted:29 November 2016
Date deposited:24 January 2017
Date of first online publication:19 January 2017
Date first made open access:No date available

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