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Construction of a chassis for a tripartite protein-based molecular motor

Small, Lara S.R.; Bruning, Marc; Thomson, Andrew R.; Boyle, Aimee L.; Davies, Roberta B.; Curmi, Paul M.G.; Forde, Nancy R.; Linke, Heiner; Woolfson, Derek N.; Bromley, Elizabeth H.C.

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Authors

Marc Bruning

Andrew R. Thomson

Aimee L. Boyle

Roberta B. Davies

Paul M.G. Curmi

Nancy R. Forde

Heiner Linke

Derek N. Woolfson



Abstract

Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and anti-parallel coiled-coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed. We use circular dichroism, analytical ultracentrifugation, dynamic light scattering and disulfide rearrangement studies to demonstrate the ability of this six-peptide set to form the specific structure required for the Tumbleweed motor. The successful formation of a suitable hub structure is both a test of the transferability of design rules for protein folding as well as an important step in the production of a synthetic protein-based molecular motor.

Citation

Small, L. S., Bruning, M., Thomson, A. R., Boyle, A. L., Davies, R. B., Curmi, P. M., …Bromley, E. H. (2017). Construction of a chassis for a tripartite protein-based molecular motor. ACS Synthetic Biology, 6(6), 1096-1102. https://doi.org/10.1021/acssynbio.7b00037

Journal Article Type Article
Acceptance Date Feb 21, 2017
Online Publication Date Feb 21, 2017
Publication Date Feb 21, 2017
Deposit Date Feb 23, 2017
Publicly Available Date Mar 28, 2024
Journal ACS Synthetic Biology
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 6
Issue 6
Pages 1096-1102
DOI https://doi.org/10.1021/acssynbio.7b00037

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Copyright Statement
This is an open access article published under a Creative Commons Attribution (CC-BY)
License, which permits unrestricted use, distribution and reproduction in any medium,
provided the author and source are cited.







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