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DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.

Cooper, L.P. and Roberts, G.A. and White, J.H. and Luyten, Y.A. and Bower, E.K.M. and Morgan, R.D. and Roberts, R.J. and Lindsay, J.A. and Dryden, D.T.F. (2017) 'DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.', Nucleic acids research., 45 (6). pp. 3395-3406.

Abstract

Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction–modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this ‘untransformable’ bacterium.

Item Type:Article
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1093/nar/gkx067
Publisher statement:© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Date accepted:20 January 2017
Date deposited:15 March 2017
Date of first online publication:09 February 2017
Date first made open access:No date available

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