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Dimer interface of Bovine cytochrome c oxidase is influenced by local posttranslational modifications and lipid binding.

Liko, I. and Degiacomi, M.T. and Mohammed, S. and Yoshikawa, S. and Schmidt, C. and Robinson, C.V. (2016) 'Dimer interface of Bovine cytochrome c oxidase is influenced by local posttranslational modifications and lipid binding.', Proceedings of the National Academy of Sciences of the United States of America., 113 (29). pp. 8230-8235.


Bovine cytochrome c oxidase is an integral membrane protein complex comprising 13 protein subunits and associated lipids. Dimerization of the complex has been proposed; however, definitive evidence for the dimer is lacking. We used advanced mass spectrometry methods to investigate the oligomeric state of cytochrome c oxidase and the potential role of lipids and posttranslational modifications in its subunit interfaces. Mass spectrometry of the intact protein complex revealed that both the monomer and the dimer are stabilized by large lipid entities. We identified these lipid species from the purified protein complex, thus implying that they interact specifically with the enzyme. We further identified phosphorylation and acetylation sites of cytochrome c oxidase, located in the peripheral subunits and in the dimer interface, respectively. Comparing our phosphorylation and acetylation sites with those found in previous studies of bovine, mouse, rat, and human cytochrome c oxidase, we found that whereas some acetylation sites within the dimer interface are conserved, suggesting a role for regulation and stabilization of the dimer, phosphorylation sites were less conserved and more transient. Our results therefore provide insights into the locations and interactions of lipids with acetylated residues within the dimer interface of this enzyme, and thereby contribute to a better understanding of its structure in the natural membrane. Moreover dimeric cytochrome c oxidase, comprising 20 transmembrane, six extramembrane subunits, and associated lipids, represents the largest integral membrane protein complex that has been transferred via electrospray intact into the gas phase of a mass spectrometer, representing a significant technological advance.

Item Type:Article
Full text:(AM) Accepted Manuscript
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Date accepted:25 May 2016
Date deposited:11 August 2017
Date of first online publication:30 June 2016
Date first made open access:11 August 2017

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