E.G. Marklund
Collision cross sections for structural proteomics
Marklund, E.G.; Degiacomi, M.T.; Robinson, C.V.; Baldwin, A.J.; Benesch, J.L.P.
Authors
Dr Matteo Degiacomi matteo.t.degiacomi@durham.ac.uk
Associate Professor
C.V. Robinson
A.J. Baldwin
J.L.P. Benesch
Abstract
Ion mobility mass spectrometry (IM-MS) allows the structural interrogation of biomolecules by reporting their collision cross sections (CCSs). The major bottleneck for exploiting IM-MS in structural proteomics lies in the lack of speed at which structures and models can be related to experimental data. Here we present IMPACT (Ion Mobility Projection Approximation Calculation Tool), which overcomes these twin challenges, providing accurate CCSs up to 106 times faster than alternative methods. This allows us to assess the CCS space presented by the entire structural proteome, interrogate ensembles of protein conformers, and monitor molecular dynamics trajectories. Our data demonstrate that the CCS is a highly informative parameter and that IM-MS is of considerable practical value to structural biologists.
Citation
Marklund, E., Degiacomi, M., Robinson, C., Baldwin, A., & Benesch, J. (2015). Collision cross sections for structural proteomics. Structure, 23(4), 791-799. https://doi.org/10.1016/j.str.2015.02.010
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 18, 2015 |
Online Publication Date | Mar 19, 2015 |
Publication Date | Apr 7, 2015 |
Deposit Date | Jul 26, 2017 |
Publicly Available Date | Aug 21, 2017 |
Journal | Structure |
Print ISSN | 0969-2126 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 23 |
Issue | 4 |
Pages | 791-799 |
DOI | https://doi.org/10.1016/j.str.2015.02.010 |
Files
Accepted Journal Article
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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/
Copyright Statement
© 2015 This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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