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Histamine type 1-receptor activation by low dose of histamine undermines human glomerular slit diaphragm integrity

Veglia, Eleonora; Pini, Alessandro; Moggio, Aldo; Grange, Cristina; Premoselli, Federica; Miglio, Gianluca; Tiligada, Katerina; Fantozzi, Roberto; Chazot, Paul L.; Rosa, Arianna Carolina

Histamine type 1-receptor activation by low dose of histamine undermines human glomerular slit diaphragm integrity Thumbnail


Authors

Eleonora Veglia

Alessandro Pini

Aldo Moggio

Cristina Grange

Federica Premoselli

Gianluca Miglio

Katerina Tiligada

Roberto Fantozzi

Arianna Carolina Rosa



Abstract

Histamine has been reported to decrease the ultrafiltration coefficient, which inversely correlates with glomerular permselectivity, however the mechanism(s) underling this effect have never been investigated. This study aimed to assess whether histamine could exert a direct detrimental effect on podocyte permeability and the possible involvement of two key proteins for the glomerular slit diaphragm (SD) integrity, zonula occludens-1 (ZO-1) and P-cadherin. The effect of histamine (100 pM–1000 nM) on coloured podocytes junctional integrity was evaluated functionally by a transwell assay of monolayer permeability and morphologically by electron microscopy. Histamine receptor (H1-4R) presence was evaluated at both mRNA (RT-PCR) and protein (immunofluorescence) levels. The Kd and Bmax values for [3H]mepyramine were determined by saturation binding analysis; IP1 and cAMP production evoked by histamine were measured by TR-FRET. ZO-1, P-cadherin and vimentin expression was assessed by qRT-PCR and quantitative immunoblotting. Histamine elicited a time- and sigmoidal dose-dependent (maximum effect at 8 h, 10 nM) increase in podocyte paracellular permeability widening the paracellular spaces. Only H1R was predominantly localised to the podocyte membrane. Consistently, histamine elicited a sigmoidal dose-dependent increase in IP1, but not in cAMP. Histamine exposure evoked a concentration-dependent reduction in both ZO-1 and P-cadherin and a parallel induction of vimentin mRNA expression with a maximum effect after 6 h, and protein expression with a maximum effect after 8 h. These effects were prevented by the selective H1R antagonist chlorpheniramine. In conclusion, our data demonstrate that histamine, via the H1R, modifies SD morphological and functional integrity, in part, by decreasing the expression of ZO-1 and P-cadherin.

Citation

Veglia, E., Pini, A., Moggio, A., Grange, C., Premoselli, F., Miglio, G., …Rosa, A. C. (2016). Histamine type 1-receptor activation by low dose of histamine undermines human glomerular slit diaphragm integrity. Pharmacological Research, 114, 27-38. https://doi.org/10.1016/j.phrs.2016.10.011

Journal Article Type Article
Acceptance Date Oct 13, 2016
Online Publication Date Oct 14, 2016
Publication Date Oct 14, 2016
Deposit Date Nov 7, 2017
Publicly Available Date Mar 28, 2024
Journal Pharmacological Research
Print ISSN 1043-6618
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 114
Pages 27-38
DOI https://doi.org/10.1016/j.phrs.2016.10.011

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