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Nanostructured titanium surfaces exhibit recalcitrance towards Staphylococcus epidermidis biofilm formation

Cao, Yunyi; Su, Bo; Chinnaraj, Subash; Jana, Saikat; Bowen, Leon; Charlton, Sam; Duan, Pengfei; Jakubovics, Nicholas S.; Chen, Jinju

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Authors

Yunyi Cao

Bo Su

Subash Chinnaraj

Saikat Jana

Leon Bowen leon.bowen@durham.ac.uk
Senior Manager (Electron Microscopy)

Sam Charlton

Pengfei Duan

Nicholas S. Jakubovics

Jinju Chen



Abstract

Titanium-based implants are ubiquitous in the healthcare industries and often suffer from bacterial attachment which results in infections. An innovative method of reducing bacterial growth is to employ nanostructures on implant materials that cause contact-dependent cell death by mechanical rupture of bacterial cell membranes. To achieve this, we synthesized nanostructures with different architectures on titanium surfaces using hydrothermal treatment processes and then examined the growth of Staphylococcus epidermidis on these surfaces. The structure obtained after a two-hour hydrothermal treatment (referred to as spear-type) showed the least bacterial attachment at short times but over a period of 6 days tended to support the formation of thick biofilms. By contrast, the structure obtained after a three-hour hydrothermal treatment (referred to as pocket-type) was found to delay biofilm formation up to 6 days and killed 47% of the initially attached bacteria by penetrating or compressing the bacteria in between the network of intertwined nano-spears. The results point to the efficacy of pocket-type nanostructure in increasing the killing rate of individual bacteria and potentially delaying longer-term biofilm formation.

Citation

Cao, Y., Su, B., Chinnaraj, S., Jana, S., Bowen, L., Charlton, S., …Chen, J. (2018). Nanostructured titanium surfaces exhibit recalcitrance towards Staphylococcus epidermidis biofilm formation. Scientific Reports, 8(1), Article 1071. https://doi.org/10.1038/s41598-018-19484-x

Journal Article Type Article
Acceptance Date Dec 27, 2017
Online Publication Date Jan 18, 2018
Publication Date Jan 18, 2018
Deposit Date Feb 8, 2018
Publicly Available Date Feb 8, 2018
Journal Scientific Reports
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Article Number 1071
DOI https://doi.org/10.1038/s41598-018-19484-x

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http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.




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