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Blobs and curves: object-based colocalisation for plant cells

Nelson, CJ; Duckney, P; Hawkins, TJ; Deeks, MJ; Laissue, PP; Hussey, PJ; Obara, B

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Authors

CJ Nelson

P Duckney

MJ Deeks

PP Laissue

PJ Hussey

B Obara



Abstract

Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.

Citation

Nelson, C., Duckney, P., Hawkins, T., Deeks, M., Laissue, P., Hussey, P., & Obara, B. (2014). Blobs and curves: object-based colocalisation for plant cells. Functional Plant Biology, 42(5), 471-485. https://doi.org/10.1071/fp14047

Journal Article Type Article
Acceptance Date Jul 21, 2014
Online Publication Date Sep 22, 2014
Publication Date Sep 22, 2014
Deposit Date Jan 28, 2015
Publicly Available Date Mar 28, 2024
Journal Functional Plant Biology
Print ISSN 1445-4408
Electronic ISSN 1445-4416
Publisher CSIRO Publishing
Peer Reviewed Peer Reviewed
Volume 42
Issue 5
Pages 471-485
DOI https://doi.org/10.1071/fp14047

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